SIH a HL5 Média

HL5 Medium including Glucose

Kód Množství Cena bez DPH

HLG0101 250g 1 250 Kč

HLG0102 1000g 3 500 Kč

HLG0103 6x1000g     17 500 Kč

HL5 Medium without Glucose

Kód Množství Cena bez DPH

HLB0101 250g 1 250 Kč

HLB0102 1000g 3 800 Kč

HLB0103 6x1000g     21 000 Kč

Popis produktu


Formula g/l


Peptone 14

Yeast Extract 7

Glucose 13.5

KH2PO4 0.5

Na2HPO4 0.5


Suspend 35.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature


Popis produktu


Formula g/l


Peptone 14

Yeast Extract 7

KH2PO4 0.5

Na2HPO4 0.5


Suspend 22 gram powdered medium in 1 litre distilled water
Store dry at room temperature


HL5-C Medium including Glucose

Kód Množství Cena bez DPH

HLC0101 250g 1 100 Kč

HLC0102 1000g 3 300 Kč

HLC0103 6x1000g 17 000 Kč

HL5-C Medium without Glucose

Kód Množství Cena bez  DPH

HLD0101 250g 1 250 Kč

HLD0102 1000g 3 800 Kč

HLD0103 6x1000g 20 000 Kč

Popis produktu


Formula g/l


Peptone 5

Yeast Extract 5

Tryptone 5

KH2PO4 1.2

Na2HPO4 0.35

Glucose 10


Suspend 26.55 gram powdered medium in 1 litre distilled water
Store dry at room temperature


Popis produktu


Formula g/l


Peptone 5

Yeast Extract 5

Tryptone 5

KH2PO4 1.2

Na2HPO4 0.35


Suspend 16.55 gram powdered medium in 1 litre distilled water
Store dry at room temperature


HL5 Medium including Glucose supplemented with vitamins and micro-elements

Kód Množství Cena bez DPH

HLE1 250g 1 250 Kč

HLE2 1000g 3 450 Kč

HLE3 6x1000g 18 500 Kč

HL5 Medium without Glucose supplemented with vitamins and micro-elements

Kód Množství Cena bez DPH

HLF1 250g 1 400 Kč

HLF2 1000g 3 700 Kč

HLF3 6x1000g 20 000 Kč

Popis produktu


Formula g/l


Peptone 14

Yeast Extract 7

Glucose 13.5

KH2PO4 0.5

Na2HPO4 0.5

FM Vitamins and Micro-elements 0.01


Suspend 35.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature


Popis produktu

HL5 Medium supplemented with vitamins and micro-elements as present in FM and SIH media.

The addition of extra vitamins and micro-elements gives good results for cultures of Dictyostelium Discoideum who require additional nutritional elements for starting up vigorous growth or protein synthesis.



Formula g/l


Peptone 14

Yeast Extract 7

KH2PO4 0.5

Na2HPO4 0.5

FM Vitamins and Micro-elements 0.01


Suspend 22 gram powdered medium in 1 litre distilled water
Store dry at room temperature


HL5-C Medium including Glucose supplemented with vitamins and micro-elements

Kód Množství Cena bez DPH

HLH1 250g 1 400 Kč

HLH2 1000g 3 500 Kč

HLH3 6x1000g 19 500 Kč

HL5-C Medium without Glucose supplemented with vitamins and micro-elements

Kód Množství Cena bez DPH

HLI1 250g 1 400 Kč

HLI2 1000g 3 700 Kč

HLI3 6x1000g 20 000 Kč

Popis produktu

HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media.

The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.



Formula g/l


Peptone 5

Yeast Extract 5

Tryptone 5

KH2PO4 1.2

Na2HPO4 0.35

Glucose 10

FM Vitamins and Micro-elements 0.01


Suspend 26.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature


Popis produktu

HL5-C supplemented with vitamins and micro-elements as present in FM and SIH media.

The addition of extra vitamins and micro-elements gives good results for cultures of Dicty who require additional nutritional elements for starting up vigorous growth or protein synthesis.



Formula g/l


Peptone 5

Yeast Extract 5

Tryptone 5

KH2PO4 1.2

Na2HPO4 0.35

FM Vitamins and Micro-elements 0.01


Suspend 16.55 gram powdered medium in 1 litre distilled water
Store dry at room temperature


SIH Medium

Kód Množství Cena bez DPH

SIH Medium SIH0101 250g 2 100 Kč

SIH Medium SIH0102 1000g 6 000 Kč

SIH Medium SIH0103 6x1000g       32 500 Kč

w/o NH4Cl SIH0501 250g 2 100 Kč

w/o NH4Cl SIH0502 1000g 6 000 Kč

w/o NH4Cl SIH0503 6x1000g        32 500 Kč

w/o Amino a. SIH0601 250g 2 100 Kč

w/o Amino a. SIH0602 1000g 6 000 Kč

w/o Amino a. SIH0603 6x1000g        32 500 Kč

w/o Amino a.

and NH4Cl SIH0701 250g 2 100 Kč

w/o Amino a.

and NH4Cl SIH0702 1000g 6 000 Kč

w/o Amino a.

and NH4Cl SIH0703 6x1000g         32 500 Kč

w/o Met SIM0101 250g 2 100 Kč

w/o Met SIM0102 1000g 6 000 Kč

w/o Met SIM0103 6x1000g         32 500 Kč

w/o Arg 

and Lys SIH1001 250g 2 100 Kč

w/o Arg 

and Lys SIH1002 1000g 6 000 Kč

w/o Arg 

and Lys SIH1003 6x1000g     32 200 Kč

w/o Cys 

and Met SIH0801 250g 2 100 Kč

w/o Cys 

and Met SIH0802 1000g 6 000 Kč

w/o Cys 

and Met SIH0803 6x1000g     32 200 Kč

w/o Glu  

and Lys SIH0901 250g 2 100 Kč

w/o Glu  

and Lys SIH0902 1000g 6 000 Kč

w/o Glu  

and Lys SIH0903 6x1000g     32 500 Kč

SIH Amino acid drop-out mixture

Kód Množství Cena bez DPH

SIH drop-out SHA02 100g 2 100 Kč

SIH drop-out SHA03 250g 4 200 Kč

minus Arg SHA05 100g 2 100 Kč

minus Arg SHA06 250g 3 800 Kč

minus Cys SHA08 100g 2 100 Kč

minus Cys SHA09 250g 3 800 Kč

minus Glu SHA11 100g 2 100 Kč

minus Glu SHA12 250g 3 800 Kč

minus Lys SHA14 100g 2 100 Kč

minus Lys SHA15 250g 3 800 Kč

minus Met SHA17 100g 2 100 Kč

minus Met SHA18 250g 3 800 Kč

minus Met 

and Cys SHA20 100g 2 100 Kč

minus met 

and Cys SHA21 250g 3 800 Kč

minus Arg 

and Glu SHA23 100g 2 100 Kč

minus Arg 

and Glu SHA24 250g 3 800 Kč

minus Arg 

and Lys SHA26 100g 2 100 Kč

minus Arg 

and Lys SHA27 250g 3 800 Kč

minus Arg 

and Met SHA29 100g 2 100 Kč

minus Arg 

and Met SHA30 250g 3 800 Kč

minus Cys 

and Glu SHA32 100g 2 100 Kč

minus Cys 

and Glu SHA33 250g 3 800 Kč

minus Cys 

and Lys SHA35 100g 2 100 Kč

minus Cys 

and Lys SHA36 250g 3 800 Kč

minus Cys 

and Met SHA38 100g 2 100 Kč

minus Cys 

and Met SHA39 250g 3 800 Kč

minus Glu 

and Lys SHA41 100g 2 100 Kč

minus Glu 

and Lys SHA42 250g 3 800 Kč

minus Glu 

and Met SHA44 100g 2 100 Kč

minus Glu 

and Met SHA45 250g 3 800 Kč

minus Lys 

and Met SHA47 100g 2 100 Kč

minus Lys 

and Met SHA48 250g 3 800 Kč

Popis produktu

SIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities.

SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977).

The main difference between SIH and FM is the novel addition of Aspartic acid (1.1 mM) and increased levels of Lysine (8.5 mM) and Tryptophane (1.7 mM). Concentrations of Cysteine, Glutamic acid, Methionine, Phenylalanine, Threonine are slightly increased as well in SIH.

The alterations in amino acid formulation of SIH medium resulted in a more even and better amino acid utilisation. Cell density of D. discoideum rose up to levels in excess of 5 x 107 cells compared to FM medium with cell density levels of 3 x 107.

Improvement of a synthetic medium for Dictyostelium discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

Cultivation of Dictostelium discoideum on an improved synthetic medium in a conventional bioreactor.
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39, 585 – 589, 2004.



Formula mg/l

Amino Acids

 

Arg 700

Asp 300

Asp A 150

Cys 300

GluA 545

Gly 900

His 300

Ile 600

Leu 900

Lys 1250

Met 350

Phe 550

Pro 800

Thr 500

Trp 350

Val 700


Vitamins

 

Biotin 0.02

Cyanocobalamin 0.01

Folic Acid 0.2

Lipoic Acid 0.4

Riboflavin 0.5

Thiamine 0.6


Micro Elements

 

Na2EDTA.2H2O 4.84

ZnSO4 2.3

H3BO3 1.11

MnCl2.4H2O 0.51

CoCl2.6H2O 0.17

CuSO4.5H2O 0.15

(NH4)6Mo7O24.4H2O 0.1


Minerals

 

NH4Cl 53.5

CaCl2.2H2O 2.94

FeCl3 16.2

MgCl2.6H2O 81.32

KH2PO4 870


Carbon Source

 

Glucose 10000


Total 20326


Suspend 20.3 gram powdered medium in 1 litre distilled water
Store dry at room temperature.


Popis produktu

SIH Amino acid drop-out mixture is based on the Amino acids present in SIH Minimal medium. These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants. Each SIH Drop-Out mixture contain all components except for one or two essential Amino acids, i.e. the “dropped out” supplements. SIH Amino acids drop-out mixtures are used in combination with SIH Minimal medium w/o Amino acids or SIH Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium.

Complete Supplement Mixture formulations are available in 25 gram and 250 gram pack sizes.

Please enquire about custom made formulations.


Complete SIH Amino acid mixture, 9195 mg/l

Formula mg/l

Arg 700

Asp 300

Asp A 150

Cys 300

GluA 545

Gly 900

His 300

Ile 600

Leu 900

Lys 1250

Met 350

Phe 550

Pro 800

Thr 500

Trp 350

Val 700

Total 9195 mg/l