Suspend 19.3 gram powdered medium in 1 litre distilled water

Store dry at room temperature 

Suspend 18.5 gram powdered medium in 1 litre distilled water

Store dry at room temperature 

Formula mg/l

Amino Acids

Arg 700

Asp 300

Cys 200

GluA 500

Gly 900

His 300

Ile 600

Leu 900

Lys 900

Met 300

Phe 500

Pro 800

Thr 500

Trp 200

Val 700

Vitamins

Biotin 0.02

Cyanocobalamin 0.01

Folic Acid 0.2

Lipoic Acid 0.4

Riboflavin 0.5

Thiamine 0.6

Micro Elements

Na2EDTA.2H2O 4.84

ZnSO4 2.3

H3BO3 1.11

MnCl2.4H2O 0.51

CoCl2.6H2O 0.17

CuSO4.5H2O 0.15

(NH4)6Mo7O24.4H2O 0.1

Minerals

NH4Cl 53.5

CaCl2.2H2O 2.94

FeCl3 16.2

MgCl2.6H2O 81.32

KH2PO4 870

Carbon Source

Glucose 10000

Total 19334.87

Suspend 19.3 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Suspend 19.3 gram powdered medium in 1 litre distilled water

Store dry at room temperature. 

Synthetic Defined Minimal Media

This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion.  Aspartic acid is added.  Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins.  Besides these components, there is a large group of amino acids present.  To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.

New to our range of synthetic media are FM and SIH without all amino acids.  These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.

Improvement of a synthetic medium for Dictyostelium Discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

Suspend 11.0 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Synthetic Defined Minimal Media

This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion.  Aspartic acid is added.  Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins.  Besides these components, there is a large group of amino acids present.  To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.

New to our range of synthetic media are FM and SIH without all amino acids.  These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.

Improvement of a synthetic medium for Dictyostelium Discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

Suspend 11.0 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Suspend 19.0 gram powdered medium in 1 litre distilled water

Store dry at room temperature. 

Suspend 17.7 gram powdered medium in 1 litre distilled water

Store dry at room temperature. 

Synthetic Defined Minimal Media

This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion.  Aspartic acid is added.  Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins.  Besides these components, there is a large group of amino acids present.  To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.

New to our range of synthetic media are FM and SIH without all amino acids.  These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.

Improvement of a synthetic medium for Dictyostelium Discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

Suspend 18.8 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Synthetic Defined Minimal Media

This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion.  Aspartic acid is added.  Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins.  Besides these components, there is a large group of amino acids present.  To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.

New to our range of synthetic media are FM and SIH without all amino acids.  These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.

Improvement of a synthetic medium for Dictyostelium Discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

Suspend 18.8 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

FM Amino acid drop-out mixture is based on the Amino acids present in FM Minimal medium. These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants. Each FM Drop-Out mixture contain all components except for one or two essential Amino acids, i.e. the “dropped out” supplements. FM Amino acids drop-out mixtures are used in combination with FM Minimal medium w/o Amino acids or FM Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium.

Complete Supplement Mixture formulations are available in 25 gram and 250 gram pack sizes.

FM Amino acid drop-out mixture is based on the Amino acids present in FM Minimal medium. These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants. Each FM Drop-Out mixture contain all components except for one or two essential Amino acids, i.e. the “dropped out” supplements. FM Amino acids drop-out mixtures are used in combination with FM Minimal medium w/o Amino acids or FM Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium. 

VL6 is a complex medium for cultivation of Discoideum dictyostelium based on Vegetable Peptone.

Formedium Ltd have recognised the need for a range of meat-free products. This has led to the development of VL6, an animal component free alternative medium compared to traditional Dicty media such as HL5 and HL5C.

VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source.  Yeast Extract is a source of vitamins, co-factors and carbohydrates. A Phosphate buffer is present to inhibit acidification of the medium during cell growth.

As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids. Essential trace elements and vitamins as present in FM and SIH media are added for those Dicty cultures needing additional nutritionals for starting up vigorous growth or protein synthesis.

Suspend 35.95g in 1L of Distilled or De-ionised Water 

FM Amino acid drop-out mixture is based on the Amino acids present in FM Minimal medium. These Amino acids form a basis for many “Drop-Out” mixtures to select for auxotrophic requirements and transformants. Each FM Drop-Out mixture contain all components except for one or two essential Amino acids, i.e. the “dropped out” supplements. FM Amino acids drop-out mixtures are used in combination with FM Minimal medium w/o Amino acids or FM Minimal medium w/o Amino acids and w/o Ammonium chloride to complete the medium.

Complete Supplement Mixture formulations are available in 25 gram and 250 gram pack sizes.

Please enquire about custom made formulations.

VL6 is a complex medium for cultivation of Discoideum dictyostelium based on Vegetable Peptone.

Formedium Ltd have recognised the need for a range of meat-free products. This has led to the development of VL6, an animal component free alternative medium compared to traditional Dicty media such as HL5 and HL5C.

VL6 is composed of a Vegetable peptone providing high molecular weight peptides and proteins as a nitrogen source. Yeast Extract is a source of vitamins, co-factors and carbohydrates. A Phosphate buffer is present to inhibit acidification of the medium during cell growth.

As most vegetable peptones contain almost no Tryptophane VL6 is supplemented with an extra quantity of this amino acid and some other amino acids. Essential trace elements and vitamins as present in FM and SIH media are added for those Dicty cultures needing additional nutritionals for starting up vigorous growth or protein synthesis.

Suspend 23.95g in 1L of Distilled or De-ionised Water