Média pro bakterie
The main components of most Esherichia Coli media are:-
Tryptone, enzymatic digest of casein.
Tryptone is a pancreatic digest of casein. Casein is the main protein of milk and is a rich source of amino acid nitrogen. Amongst all the amino acids, Tryptophan is present in the highest concentrations.
Due to the rich nutritional properties, Tryptone is added to media as an accelerator to increase the yield of organisms and is recommended where a rapid and luxuriant growth of micro organisms is required.
Yeast Extract
Yeast Extract is a spray dried extract manufactured by complete autolysis, i.e. a transformation of proteins into peptides and amino acids, implemented through the proteolytic enzymes present in yeast cells.
The cell membranes are discarded, enabling completely soluble yeast extracts to be obtained. In LB media, Yeast Extract is an essential nitrogen source for bacteria besides peptides and amino acids. Yeast extract also contains purine and pyrimidine bases, carbohydrates and water soluble vitamins of B group.
Glucose
As the main carbon source
Sodium Chloride
To set the osmolarity of the medium at a suitable osmotic environment.
Agar
When added, to solidify the medium.
Additives
Like Casamino acids, Magnesium sulfate, Maltose, Thymidine.
In fact most Escherichia Coli media do contain these components, but all in different compositions and ratios to obtain an optimal result for cell growth and yield, propagation of bacteriophages and preparation of competent cells.
Téměř veškerá média lze dodat i s přídavkem agaru (cena na požádání)
LB w/o NaCl
Kód Množství Cena bez DPH
LBO0101 250g 1 250 Kč
LBO0102 1000g 3 500 Kč
LBO0103 6x1000g 17 500 Kč
Sabouraud Broth
Kód Množství Cena bez DPH
SAB0202 250g 700 Kč
SAB0205 500g 1 100 Kč
SAB0210 1000g 2 000 Kč
Popis produktu
Formula g/l
Tryptone 10
Yeast extract 5
Final pH 7.0 ± 0.2 at 25°C
Suspend 15gram powdered medium in 1 litre distilled water
Popis produktu
Final pH 7.0 ± 0.2 at 25°C
Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature
L-Broth
Kód Množství Cena bez DPH
LBL0101 250g 1 000 Kč
LBL0102 1000g 2 250 Kč
LBL0103 6x1000g 10 500 Kč
VLB-Broth Lennox
Kód Množství Cena bez DPH
VLBBL0250 250g 700 Kč
VLBBL0500 500g 1 350 Kč
VLBBL1000 1000g 2 500 Kč
Popis produktu
Luria Broth Base (Miller’s Modification)
Luria Broth Base or L-Broth is based upon the Luria Broth formulation as originally developed by Miller for cultivation and maintenance of E. coli cells in molecular biology.
L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize, support a luxurious growth of E. coli cells.
Sodium ions for transport and osmotic balance are provided by Sodium chloride.
The concentration of NaCl in L-Broth is low compared to both LB Miller and LB Lennox formulations, respectively 10% and 5% of the NaCl concentration is present in both formulations.
These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain.
Miller, J.H., Experiments in molecular genetics, Cold Spring harbour Laboratory, Cold Spring harbour, New York, (1972).
Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955).
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
Popis produktu
Formula g/l
Formula g/l
Soya Peptone 10
Yeast extract 5
Final pH 7.0 ± 0.2 at 25°C
NaCl 5
LB-Broth Lennox
Kód Množství Cena bez DPH
LBX0101 250g 1 100 Kč
LBX0104 500g 1 650 Kč
LBX0102 1000g 2 750 Kč
LBX0103 6x1000g 14 250 Kč
VLB-Broth Miller
Kód Množství Cena bez a DPH
VLBBM0250 250g 800 Kč
VLBBM0500 500g 1 400 Kč
VLBBM1000 1000g 2 500 Kč
VLBBM6000 6x1000g 13 500 Kč
Popis produktu
LB-Broth Low Salt, Lennox L Broth
LB Broth Lennox is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. E. coli is grown to late log phase in LB Broth. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. K12 has been ultimately modified by specific mutation into an auxothropic strain not capable of growth on nutritionally deficient medium. All nutritional requirements of E. coli strains are provided by LB Broth. Peptides and amino acids are abundantly present in Tryptone. Yeast extract is a rich source of amino acids, vitamins, nucleotides and carbohydrates. These nutritional elements in LB media, which otherwise the cell would have to synthesize, support a luxurious growth of E. coli cells.
Sodium ions for transport and osmotic balance are provided by Sodium chloride. The concentration of NaCl in LB Lennox is half the concentration of the LB Miller Broth formulation and ten times the concentration of LB Luria Broth formulation. These variations in Sodium chloride content make it possible to select the optimal salt concentration for a specific strain.
Lennox, E.S., Transuction of linked genetic characters of the host by bacteripphage P1, 1, 190-206, (1955).
Assubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl, Current protocols in molecular biology, vol. 1, Current Protocols, New York, (1994).
Formula g/l
Soya Peptone 10
Yeast extract 5
Final pH 7.0 ± 0.2 at 25°C
NaCl 5
Popis produktu
Formula g/l
Soya Peptone 10
Yeast extract 5
Final pH 7.0 ± 0.2 at 25°C
NaCl 10
LB-Broth Miller
Kód Množství Cena bez DPH
LMM0101 250g 900 Kč
LMM0104 500g 1 450 Kč
LMM0102 1000g 2 700 Kč
LMM0103 6x1000g 15 000 Kč
Blood Agar Base
Kód Množství Cena bez DPH
BAB0105 500g 2 500 Kč
BAB0110 1000g 4 250 Kč
BAB0610 6x1000g 23 500 Kč
Popis produktu
Formula g/l
Peptone 10
Yeast extract 5
Final pH 7.0 ± 0.2 at 25°C
NaCl 10
Popis produktu
Formula g/l
Peptone 10
Beef Extract 10
NaCl 5
Agar 12
Final pH 7.0 ± 0.2 at 25°C
Blood Agar Base is a non-selective general purpose medium widely employed for the growth of pathogenic and non-pathogenic bacteria
Suspend 37gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Mueller Hinton Broth
Kód Množství Cena bez DPH
MHB0100 250g 1 750 Kč
MHB0101 1000g 4 500 Kč
MHB0102 6x1000g 25 000 Kč
Terrific Broth
Kód Množství Cena bez DPH
TRB0101 250g 850 Kč
TRB0104 500g 1 500 Kč
TRB0102 1000g 2 850 Kč
TRB0103 6x1000g 15 500 Kč
Popis produktu
Formula g/l
Casamino acids 17.5
Peptone 2
Soluble starch 1.5
Final pH 7.0 ± 0.2 at 25°C
Suspend 21 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Popis produktu
Terrific Broth is rich nutritional medium developed by Tartoff and Hobbs supporting luxurious high density growth of E.coli and a higher yield of plasmid DNA compared to LB type media. Recombinant E. coli cells have an extended growth phase in Terrific medium.
Terrific Broth medium is used with glycerol as a carbon source. Unlike glucose, glycerol is not fermented to acetic acid.
Tryptone and Yeast are present in the medium in elevated concentrations and provide nitrogen, vitamins and cofactors for a luxurious growth.
Potassium phosphate is present in Terrific broth buffered to balance the pH and subsequently prevent cell death due to a drop in pH.
Tartoff, K. D., and C. A. Hobbs, Improved media for growing plasmids and cosmid clones, Bethesda Research Laboratories, 9, 12, (1987).
Sambrook, J,., E. F. Fritsch, and T. Maniatis, 1989, Molecular cloning: a laboratory manual, 2nd edition ed., Cold Spring Harbour laboratory, Cold Spring Harbour, N.Y.
Formula g/l
Tryptone 12
Yeast Extract 24
Final pH 7.0 ± 0.2 at 25°C
Suspend 36 gram powdered medium in 1 litre distilled water
Add 4ml Glycerol
Store dry at room temperature.
YT Broth
Kód Množství Cena bez DPH
YTB0101 250g 867 Kč
YTB0102 1000g 2 800 Kč
YTB0103 6x1000g 15 500 Kč
NZ Broth
Kód Množství Cena bez a DPH
NZB0101 250g 1 000 Kč
NZB0102 1000g 2 300 Kč
Popis produktu
Formula g/l
Tryptone 8
Yeast Extract 5
NaCl 5
Final pH 7.0 ± 0.2 at 25°C
Suspend 18 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Popis produktu
Formula g/l
Tryptone 10
MgSO4 anhydrous 0.98
NaCl 5
Final pH 7.0 ± 0.2 at 25°C
Suspend 16 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
NZCYM Broth
Kód Množství Cena bez DPH
NZC0101 250g 1 000 Kč
NZC0102 1000g 2 500 Kč
NZC0103 6x1000g 14 500 Kč
SOB
Kód Množství Cena bez DPH
SOB0101 250g 1 000 Kč
SOB0102 1000g 3 100 Kč
Popis produktu
Formula g/l
Casamino acids 1
Tryptone 10
Yeast Extract 5
NaCl 5
MgSO4 anhydrous 0.98
Final pH 7.0 ± 0.2 at 25°C
Suspend 22 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Popis produktu
Formula g/l
Tryptone 10
Yeast Extract 5
NaCl 0.5
MgSO4 2.44
Final pH 7.0 ± 0.2 at 25°C
Suspend 27.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
SOC Broth
Kód Množství Cena bez DPH
SOC0201 250g 900 Kč
SOC0202 1000g 2 500 Kč
AIM - LB Broth Base w/o Trace elements
Kód Množství Cena bez DPH
AIMLB0101 100g 750 Kč
AIMLB0105 500g 2 000 Kč
AIMLB0110 1000g 3 400 Kč
AIMLB0160 6x1000g 18 000 Kč
Popis produktu
Formula g/l
Glucose 3.6 (20 mN)
Tryptone 20
Yeast Extract 5
NaCl 0.5
MgSO4 2.44
Final pH 7.0 ± 0.2 at 25°C
Suspend 31.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Popis produktu
Formula g/l
Tryptone 10
Yeast Extract 5
(NH4)2SO4 3.3
KH2PO4 6.8
Na2HPO4 7.1
Glucose 0.5
MgSO4 0.15
alpha-Lactose 2.0
Suspend 34.85 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Tryptic Soy Broth
Kód Množství Cena bez DPH
TSB0110 1000g 2 200 Kč
AIM - LB Broth Base including Trace elements
Kód Množství Cena bez DPH
AIMLB0201 100g 750 Kč
AIMLB0205 500g 2 050 Kč
AIMLB0210 1000g 3 500 Kč
AIMLB0260 6x1000g 18 500 Kč
Popis produktu
Formula Supplement g/l (%w/w)
Tryptone 17 (56.67)
Soya Peptone A3 3 (10)
Glucose 2.5 (8.33)
Di-Potassium Hydrogen Phosphate 2.5 (8.33)
Sodium Chloride 5 (16.67)
Suspend 30g in 1L of Distilled or De-ionised Water
Store dry at room temperature
Popis produktu
Formula g/l
Tryptone 10
Yeast Extract 5
(NH4)2SO4 3.3
KH2PO4 6.8
Na2HPO4 7.1
Glucose 0.5
MgSO4 0.15
alpha-Lactose 2.0
Trace Elements 0.03
Suspend 34.85 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Lee´s Medium
Kód Množství Cena bez DPH
LEES0500 500g 5 500 Kč
LEES1000 1000g 10 000 Kč
LoFlo Medium
Kód Množství Cena bez DPH
LF0501 500g 2 500 Kč
LF1001 1000g 4 250 Kč
LF6001 6x1000g 22 000 Kč
Popis produktu
Formula g/l
(NH4)2SO4 5.0
MgSO4.7H20 0.2
K2HPO 2.5
NaCl 5.0
L-Alanine 0.5
L-Leucine 1.3
L-Lysine 1.0
L-Methionine 0.1
L-Ornithine 0.0714
L-Phenylalanine 0.5
L-Proline 0.5
L-Threonine 0.5
Biotin 0.001
Suspend 17.09g in 1L of Distilled or De-ionised Water
HYGROSCOPIC Store at Room Temperature
DO NOT BREATHE DUST Keep Container Tightly Closed
Popis produktu
Formula g/l
Glucose 11
KH2PO4 0.68
Casein peptone 5
NH4Cl 26.8
MgCl2 37.1
CaCl2 1.1
FeCl3 8.11
NaA-EDTA 4.84
ZnSO4 2.30
H3BO3 1.11
MnCl2.4H2O 0.51
CoCl2 0.17
CuSO5.5H2O 0.15
(NH4)6Mo7O24.4H2O 0.1
Suspend 16.8 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Adjust to pH 6.5
LoFlo Medium supplemented with Yeast extract
Kód Množství Cena bez DPH
LFG0501 500g 2 500 Kč
LFG1001 1000g 4 200 Kč
LFG6001 6x1000g 22 000 Kč
Vegetal LoFlo Medium
Kód Množství Cena bez DPH
VLF0105 500g 2 500 Kč
VLF0110 1000g 4 250 Kč
VLF0610 6x1000g 22 000 Kč
Popis produktu
Formula g/l
Glucose 11
KH2PO4 0.68
Casein peptone 5
Yeast extract 0,7
NH4Cl 26.8
MgCl2 37.1
CaCl2 1.1
FeCl3 8.11
NaA-EDTA 4.84
ZnSO4 2.30
H3BO3 1.11
MnCl2.4H2O 0.51
CoCl2 0.17
CuSO5.5H2O 0.15
(NH4)6Mo7O24.4H2O 0.1
Suspend 17.5 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Adjust to pH 6.5
Popis produktu
LoFlo vs VLoFlo:
Triplicate biological repeats (cultures)
Cell counts made to 48 h
Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo
By 47 h cell rounding and shrinkage pronounced and assay stopped
* Noted previously in Dicty community that LoFlo is not ideal for long-term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to <24 h but allowing HL-5-containing endosomes to disappear, reducing autofluorescence.
M17 Broth
Kód Množství Cena bez DPH
M170110 1000g 4 600 Kč
M170610 6x1000g 24 000 Kč
Popis produktu
M17 Medium is a well buffered enriched medium for bacterial growth. The medium is optimized for isolating lactic Streptococci. Casein hydrolysate, Beef extract and Soya peptone supply carbon sources, nitrogen, vitamins and minerals essential for vigorous growth. Yeast extract supplies B-complex vitamins which stimulate bacteria growth. The medium is buffered by Disodium-β-glycerophosphate which prevents a drop in pH by the production of organic acids, such as lactic acid, during fermentation.
Ascorbic acid and Magnesium sulphate are added to stimulate growth.
Formula g/l
Pancreatic Digest of Casein 5.0
Soy Peptone 5.0
Beef Extract 5.0
Yeast Extract 2.5
Ascorbic Acid 0.5
Magnesium sulfate 0.25
Disodiumglycerophosphate 19.0
Suspend 36.15 grams of powdered medium in 1 litre distilled water
Store dry at room temperature.