Média pro kvasinky
All media are formulated as described in the Cold Spring Harbor Lab Manual for Yeast Genetics, Kaiser, C., et al., Methods in Yeast Genetics, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994) and the Difco Manual 11th ed.
Téměř veškerá média lze dodat i s přídavkem agaru.
Možnost dodat i různé modifikace YNB, SD a MD médií:
Složení Yeast nitrogen base médií, Synthetic defined (SD) médií a Minimal Defined (MD) médií
Yeast nitrogen base
Kód Množství Cena bez DPH
CYN0201 100g 1 700 Kč
CYN0202 250g 2 500 Kč
CYN0205 500g 4 350 Kč
CYN0210 1000g 8 250 Kč
Yeast nitrogen base without amino acid
Kód Množství Cena bez DPH
CYN0401 100g 1200 Kč
CYN0402 250g 2 600 Kč
CYN0405 500g 4 400 Kč
CYN0410 1000g 8 350 Kč
Popis produktu
Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Yeast Nitrogen Base is based upon the formulation of Yeast Agar except that Ammonium Sulfate is the sole nitrogen source and that addition of carbon source is required. The medium may be used to test carbon assimilation in the presence of a carbon source like glucose, galactose or raffinose
Popis produktu
Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Yeast Nitrogen Base without amino acids (YNB w/o AA) is used for selecting yeasts based on amino acid and carbohydrate requirements. In Yeast Nitrogen Base with out amino acids Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells.
Yeast carbon base
Kód Množství Cena bez DPH
CYN0601 100g 1 250 Kč
CYN0602 250g 2 500 Kč
CYN0605 500g 3 850 Kč
CYN0610 1000g 7 250 Kč
Yeast morphology agar
Kód Množství Cena bez DPH
CYN0101 100g 2 000 Kč
CYN0102 250g 3 250 Kč
CYN0105 500g 5 500 Kč
CYN0110 1000g 10 000 Kč
Popis produktu
Suspend 11.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Yeast Carbon Base without Nitrogen source is used to test the ability of yeasts to assimilate nitrogen by the addition of various nitrogen sources. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base.
Popis produktu
Suspend 35.0 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Yeast Agar is a rich well defined medium including Ammonium Sulfate and Asparagine as a nitrogen source and is used for typical colonial morphology. The medium provides a carbon- and nitrogen source in sufficient concentrations including amino acids, vitamins, trace elements and salts resulting in a good development of yeast colonies.
Yeast mold broth
Kód Množství Cena bez DPH
YMBR0105 500g 2 250 Kč
YMBR0110 1000g 3 000 Kč
YMBR1000 10*1000g 22 000 Kč
Yeast extract powder
Kód Množství Cena bez DPH
YEA01 250g 750 Kč
YEA02 500g 1 250 Kč
YEA03 1000g 2 150 Kč
YEA06 2000g 4 050 Kč
Popis produktu
Suspend 21 powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast Extract Powder 3
Malt Extract 3
Peptone 5
Dextrose 10
Final pH 7.0 ± 0.2 at 25°C
Popis produktu
Yeast Extract is a spray dried extract manufactured by complete autolysis, i.e. a transformation of proteins into peptides, and amino acids, implemented through the proteolytic enzymes present in yeast cells.
The cell membranes are discarded, enabling completely soluble yeast extracts to be obtained. Besides peptides and amino acids yeast extract also contains purine and pyrimidine bases, carbohydrates and water soluble vitamins of B group.
Sodium Chloride concentration of Formedium™Yeast Extract is low and also therefore well suited for cultivation of yeast cells.
Due to its carbohydrate content, typically 10%, yeast extract is not suitable for media intended for the study of sugar fermentation.
Store dry at room temperature
Yeast vitamin free base
Kód Množství Cena bez DPH
CYN0701 100g 1 250 Kč
CYN0702 250g 2 800 Kč
CYN0705 500g 4 750 Kč
CYN0710 1000g 8 500 Kč
YPD broth
Kód Množství Cena bez DPH
CCM0202 250g 1 100 Kč
CCM0205 500g 1 400 Kč
CCM0210 1000g 2 600 Kč
CCM0260 6*1000g 13 000 Kč
Popis produktu
Suspend 17.0 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Yeast Nitrogen Base Vitamin Free is based upon the formulation of Yeast Nitrogen base except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins.
Popis produktu
Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Glucose 20
YEP broth
Kód Množství Cena bez DPH
CCM0402 250g 1 250 Kč
CCM0405 500g 1 850 Kč
CCM0410 1000g 3 500 Kč
YPAD broth
Kód Množství Cena bez DPH
CCM1002 250g 1 200 Kč
CCM1005 500g 2 000 Kč
CCM1010 1000g 3 300 Kč
Popis produktu
Suspend 30 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Popis produktu
Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Glucose 20
Adenine Sulfate 0.04
YPL Broth with lactose
Kód Množství Cena bez DPH
YPLB01 250g 1 250 Kč
YPLB02 500g 2 000 Kč
YPLB03 1000g 3 200 Kč
MAL indicator medium
Kód Množství Cena bez DPH
CCM0602 250g 1 400 Kč
CCM0605 500g 2 400 Kč
CCM0610 1000g 4 250 Kč
Popis produktu
Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Lactose 20
Adenine Sulfate 0.04
Popis produktu
Suspend 70 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Maltose 20
Agar 20
GAL indicator medium
Kód Množství Cena bez DPH
CCM0702 250g 1 700 Kč
CCM0705 500g 3 300 Kč
CCM0710 1000g 5 250 Kč
Sporulation medium
Kód Množství Cena bez DPH
CCM0802 250g 1 600 Kč
CCM0805 500g 2 400 Kč
CCM0810 1000g 4 600 Kč
Popis produktu
Suspend 70 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Galactose 20
Agar 20
Popis produktu
Suspend 40.2 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Glucose 0.2
Potassium Acetate 10
Agar 20
Pre-spoluration medium
Kód Množství Cena bez DPH
CCM0902 250g 600 Kč
CCM0905 500g 900 Kč
CCM0910 1000g 1 400 Kč
Malt extract
Kód Množství Cena bez DPH
MAL03 1000g 2 900 Kč
Popis produktu
Suspend 150 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Glucose 100
Potassium Acetate 20
Agar 20
Popis produktu
Solubility in water at 3 % Complete
pH (3 % solution) 4.8 – 5.8
Loss on drying ≤ 6.0 %
Reducing sugars (as maltose) ≥ 60.0 %
Residue on ignition ≤ 4.5 %
Chloride (as NaCl) ≤ 1.0 %
Store dry at room temperature
Soya peptone
Kód Množství Cena bez DPH
VPEP01 250g 1 075 Kč
VPEP02 500g 1 950 Kč
VPEP03 1000g 3 500 Kč
VPEP04 6*1000g 18 500 Kč
Peptone
Kód Množství Cena bez DPH
PEP01 250g 1 400 Kč
PEP02 500g 2 250 Kč
PEP03 1000g 4 250 Kč
PEP04 6*1000g 23 500 Kč
Popis produktu
Soya Peptone is a papaic digest of defatted soybean flour and is a well-balanced source of essential amino acids, carbohydrates and vitamins in cell cultures.
Soya Peptone is used for growth of a wide variety of bacteria and yeasts in cell cultures and is often combined with Tryptone or Peptone for a rapid and abundant growth of cells.
This plant peptone is classified animal-free by Formedium Ltd. Based on the manufacturing protocol, we attest that no animal raw materials are prescribed for use in the production of this product, nor are any of the raw materials derived from animal products.
Soya Peptone is classified animal free, GMO free (according to the European Directive 2001/18/CE)
Store dry at room temperature
Popis produktu
Peptone is a spray dried powder, manufactured by a controlled enzymatic hydrolysis of animal tissue. The most commonly used enzymes are pepsin, papain and pancreatin. The latter containing trypsin.
Pepsin will cut the peptide chain anywhere there is a phenylalanine or leucine bond.
Papain cuts in the peptide chain adjacent to arginine, lysine, phenylalanine and glycine. Pancreatin has its action at arginine, lysine, tyrosine, tryptophan, phenylalanine and leucine bonds.
The tissues are hydrolysed to produce straw coloured peptones which are highly nutritious and clearly soluble in water. Peptones contain a mix of peptides, free amino acids and growth factors.
Due to the low sodium chloride concentration Formedium™Pepton is well suited for cultivation of yeast cells.
Store dry at room temperature
CAS Number 73049-73-7
PDB
Kód Množství Cena bez DPH
PDB0101 100g 500 Kč
PDB0102 1000g 2 900 Kč
PDB0103 5000g 12 000 Kč
PDA
Kód Množství Cena bez DPH
PDA0101 100g 750 Kč
PDA0102 1000g 3 250 Kč
PDA0103 5000g 14 000 Kč
Popis produktu
Suspend 24 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Formula g/l
Potato extrakt 4
Glucose 20
Popis produktu
Suspend 41 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Potato extrakt 4
Glucose 20
Agar 17
Potato extract
Kód Množství Cena bez DPH
PTE01 250g 1 500 Kč
PTE02 500g 2 200 Kč
PTE03 1000g 3 500 Kč
PTE04 6*1000g 18 500 Kč
Casamino acids
Kód Množství Cena bez DPH
CAS01 250g 1 800 Kč
CAS02 500g 2 700 Kč
CAS03 1000g 5 400 Kč
CAS04 6x1000g 29 750 Kč
Popis produktu
Potato extract is a mixture of potato proteins, manufactured by controlled enzymatic hydrolysis. The extract is an excellent nitrogen source for bacteria, yeasts and fungi. Potato extract is rich in vitamins and minerals and supports a vigorous growth of micro-organisms.
Store dry at room temperature
Popis produktu
Casamino Acids are manufactured by a controlled acid hydrolysis of casein . Hydrolysis is not completed until all the nitrogen in the casein is converted to amino acids or other compounds of relative chemical simplicity. As a result of the acid hydrolysis process all vitamins and growth factors present in casein are destroyed.
Due to the low sodium chloride concentration Formedium™Casamino Acids are well suited for cultivation of yeast cells.
Tryptone
Kód Množství Cena bez DPH
TRP01 250g 1 400 Kč
TRP02 500g 2 400 Kč
TRP03 1000g 4 200 Kč
TRP04 6*1000g 23 500 Kč
Yeast potassium nitrate nitrogen base
Kód Množství Cena bez DPH
CYN0301 100g 1 700 Kč
CYN0302 250g 3 400 Kč
CYN0305 500g 6 100 Kč
CYN0310 1000g 9 800 Kč
Popis produktu
Store dry at room temperature
CAS 91079-40-2
Popis produktu
Suspend 2.7 gram powdered medium in 1 litre distilled water
Store dry at room temperature
Yeast Potassium Nitrate Nitrogen Base contains Potassium Nitrate as a sole nitrogen source to test nitrogen assimilation. The addition of a carbon source is required. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base.
Yeasts which have grown on a rich medium like Yeast Agar may carry a reserve of nitrogen in the form of protein. To avoid errors due to this reserve of nitrogen it is advised to make two serial transfers in the medium. Seven days after the first inoculation the second inoculation is made in the same medium. If a positive result is obtained after seven days, the yeast cells assimilates nitrate as sole nitrogen source.
SD broth 2% glucose
Kód Množství Cena bez DPH
CSM0201 100g 1 250 Kč
CSM0202 250g 2 300 Kč
CSM0205 500g 4 300 Kč
CSM0210 1000g 7 800 Kč
SD broth 2% galactose
Kód Množství Cena bez DPH
CSM0401 100g 2 200 Kč
CSM0402 250g 3 900 Kč
CSM0405 500g 7 500 Kč
CSM0410 1000g 14 500 Kč
Popis produktu
Suspend 26.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
SD Broth / Glucose containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.
Popis produktu
Suspend 26.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
SD Broth / Galactose containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.
SD broth 2% raffinose
Kód Množství Cena bez DPH
CSM0601 100g 2 800 Kč
CSM0602 250g 5 500 Kč
CSM0605 500g 11 000 Kč
CSM0610 1000g 20 000 Kč
SD broth 3.3% succinate
Kód Množství Cena bez DPH
CSM0801 100g 1 250 Kč
CSM0802 250g 2 500 Kč
CSM0805 500g 4 500 Kč
CSM0810 1000g 8 500 Kč
Popis produktu
Suspend 26.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
SD Broth / Raffinose containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.
Popis produktu
Suspend 57.9 gram powdered medium in 1 litre distilled water
SD Broth / Succinate containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.
SD broth 2% glucose
without phosphate KH2PO4
Kód Množství Cena bez DPH
CSM1201 100g 1 250 Kč
CSM1202 250g 2 250 Kč
CSM1205 500g 4 000 Kč
CSM1210 1000g 7 500 Kč
SD broth 2% galactose
1% raffinose
Kód Množství Cena bez DPH
CSM1001 100g 2 600 Kč
CSM1002 250g 5 600 Kč
CSM1005 500g 9 500 Kč
CSM1010 1000g 16 750 Kč
Popis produktu
Suspend 25.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
SD Broth / 2% Glucose w/o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose. The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.
Popis produktu
Suspend 36.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature
SD Broth / Galactose and Raffinose containing Yeast Nitrogen base without Amino Acids, galactose and raffinose. Agar has been omitted from the original formulation.
YPD Agar
Kód Množství Cena bez DPH
CCM0102 250g 1 200 Kč
CCM0105 500g 1 600 Kč
CCM0110 1000g 2 900 Kč
YEP Agar
Kód Množství Cena bez DPH
CCM0302 250g 1 200 Kč
CCM0305 500g 2 100 Kč
CCM0310 1000g 3 500 Kč
Popis produktu
Suspend 70 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Glucose 20
Agar 20
Popis produktu
Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
YEP Medium is based upon YPD but is without dextrose and can be used as a base for making YPD media with an alternate carbon source.
To make YEPG-Medium add 38 ml glycerol per litre YEP medium. Glycerol is a non-fermentable carbon source. Respiratory deficient and pet mutants will not grow on YPEG.
Formula g/l
Yeast extract 10
Peptone 20
Agar 20
YPAD Agar
Kód Množství Cena bez DPH
CCM0502 250g 1 200 Kč
CCM0505 500g 2 100 Kč
CCM0510 1000g 3 500 Kč
YPL Agar with Lactose
Kód Množství Cena bez DPH
YPLA01 250g 1 200 Kč
YPLA02 500g 2 400 Kč
YPLA03 1000g 3 700 Kč
Popis produktu
Suspend 70 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Glucose 20
Adenine Sulfate 0.04
Agar 20
Popis produktu
Suspend 70 gram powdered medium in 1 litre distilled water
Store dry at room temperature.
Formula g/l
Yeast extract 10
Peptone 20
Lactose 20
Adenine Sulfate 0.04
Agar 20