Média pro kvasinky

All media are formulated as described in the Cold Spring Harbor Lab Manual for Yeast Genetics, Kaiser, C., et al., Methods in Yeast Genetics, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1994) and the Difco Manual 11th ed.

Téměř veškerá média lze dodat i s přídavkem agaru.

Možnost dodat i různé modifikace YNB, SD a MD médií:

Složení Yeast nitrogen base médií, Synthetic defined (SD) médií a Minimal Defined (MD) médií

Yeast nitrogen base

Kód Množství Cena bez DPH

CYN0201 100g 1 700 Kč

CYN0202 250g 2 500 Kč

CYN0205 500g 4 350 Kč

CYN0210 1000g 8 250 Kč

Yeast nitrogen base without amino acid

Kód Množství Cena bez DPH

CYN0401 100g 1200 Kč

CYN0402 250g 2 600 Kč

CYN0405 500g 4 400 Kč

CYN0410 1000g 8 350 Kč

Popis produktu

Suspend 6.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Yeast Nitrogen Base is based upon the formulation of Yeast Agar except that Ammonium Sulfate is the sole nitrogen source and that addition of carbon source is required. The medium may be used to test carbon assimilation in the presence of a carbon source like glucose, galactose or raffinose

Popis produktu

Suspend 6.9 gram powdered medium in 1 litre distilled water

Store dry at room temperature

Yeast Nitrogen Base without amino acids (YNB w/o AA) is used for selecting yeasts based on amino acid and carbohydrate requirements. In Yeast Nitrogen Base with out amino acids Ammonium sulphate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells.

Yeast carbon base

Kód Množství Cena bez DPH

CYN0601 100g 1 250 Kč

CYN0602 250g 2 500 Kč

CYN0605 500g 3 850 Kč

CYN0610 1000g 7 250 Kč

Yeast morphology agar

Kód Množství Cena bez DPH

CYN0101 100g 2 000 Kč

CYN0102 250g 3 250 Kč

CYN0105 500g 5 500 Kč

CYN0110 1000g 10 000 Kč

Popis produktu

Suspend 11.9 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Yeast Carbon Base without Nitrogen source is used to test the ability of yeasts to assimilate nitrogen by the addition of various nitrogen sources. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base.

Popis produktu

Suspend 35.0 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Yeast Agar is a rich well defined medium including Ammonium Sulfate and Asparagine as a nitrogen source and is used for typical colonial morphology. The medium provides a carbon- and nitrogen source in sufficient concentrations including amino acids, vitamins, trace elements and salts resulting in a good development of yeast colonies.

Yeast mold broth

Kód Množství Cena bez DPH

YMBR0105 500g 2 250 Kč

YMBR0110 1000g 3 000 Kč

YMBR1000 10*1000g 22 000 Kč

Yeast extract powder

Kód Množství Cena bez DPH

YEA01 250g 750 Kč

YEA02 500g 1 250 Kč

YEA03 1000g 2 150 Kč

YEA06 2000g 4 050 Kč

Popis produktu

Suspend 21 powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast Extract Powder 3

Malt Extract 3

Peptone 5

Dextrose 10

Final pH 7.0 ± 0.2 at 25°C

Popis produktu

Yeast Extract is a spray dried extract manufactured by complete autolysis, i.e. a transformation of proteins into peptides, and amino acids, implemented through the proteolytic enzymes present in yeast cells.

The cell membranes are discarded, enabling completely soluble yeast extracts to be obtained. Besides peptides and amino acids yeast extract also contains purine and pyrimidine bases, carbohydrates and water soluble vitamins of B group.

Sodium Chloride concentration of Formedium™Yeast Extract is low and also therefore well suited for cultivation of yeast cells.

Due to its carbohydrate content, typically 10%, yeast extract is not suitable for media intended for the study of sugar fermentation.

Store dry at room temperature

Yeast vitamin free base

Kód Množství Cena bez DPH

CYN0701 100g 1 250 Kč

CYN0702 250g 2 800 Kč

CYN0705 500g 4 750 Kč

CYN0710 1000g 8 500 Kč

YPD broth

Kód Množství Cena bez DPH

CCM0202 250g 1 100 Kč

CCM0205 500g 1 400 Kč

CCM0210 1000g 2 600 Kč

CCM0260 6*1000g 13 000 Kč

Popis produktu

Suspend 17.0 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Yeast Nitrogen Base Vitamin Free is based upon the formulation of Yeast Nitrogen base except that all vitamins are omitted. The medium is used to test the requirement of essential vitamins.

Popis produktu

Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Glucose 20

YEP broth

Kód Množství Cena bez DPH

CCM0402 250g 1 250 Kč

CCM0405 500g 1 850 Kč

CCM0410 1000g 3 500 Kč

YPAD broth

Kód Množství Cena bez DPH

CCM1002 250g 1 200 Kč

CCM1005 500g 2 000 Kč

CCM1010 1000g 3 300 Kč

Popis produktu

Suspend 30 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Popis produktu

Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Glucose 20

Adenine Sulfate 0.04

YPL Broth with lactose

Kód Množství Cena bez DPH

YPLB01 250g 1 250 Kč

YPLB02 500g 2 000 Kč

YPLB03 1000g 3 200 Kč

MAL indicator medium

Kód Množství Cena bez DPH

CCM0602 250g 1 400 Kč

CCM0605 500g 2 400 Kč

CCM0610 1000g 4 250 Kč

Popis produktu

Suspend 50 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Lactose 20

Adenine Sulfate 0.04

Popis produktu

Suspend 70 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Maltose 20

Agar 20

GAL indicator medium

Kód Množství Cena bez DPH

CCM0702 250g 1 700 Kč

CCM0705 500g 3 300 Kč

CCM0710 1000g 5 250 Kč

Sporulation medium

Kód Množství Cena bez DPH

CCM0802 250g 1 600 Kč

CCM0805 500g 2 400 Kč

CCM0810 1000g 4 600 Kč

Popis produktu

Suspend 70 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Galactose 20

Agar 20

Popis produktu

Suspend 40.2 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Glucose 0.2

Potassium Acetate 10

Agar 20

Pre-spoluration medium

Kód Množství Cena bez DPH

CCM0902 250g 600 Kč

CCM0905 500g 900 Kč

CCM0910 1000g 1 400 Kč

Malt extract

Kód Množství Cena bez DPH

MAL03 1000g 2 900 Kč

Popis produktu

Suspend 150 gram powdered medium in 1 litre distilled water
Store dry at room temperature.

Formula g/l

Yeast extract 10

Glucose 100

Potassium Acetate 20

Agar 20

Popis produktu

Solubility in water at 3 % Complete
pH (3 % solution) 4.8 – 5.8
Loss on drying ≤ 6.0 %
Reducing sugars (as maltose) ≥ 60.0 %
Residue on ignition ≤ 4.5 %
Chloride (as NaCl) ≤ 1.0 %

Store dry at room temperature

Soya peptone

Kód Množství Cena bez DPH

VPEP01 250g 1 075 Kč

VPEP02 500g 1 950 Kč

VPEP03 1000g 3 500 Kč

VPEP04 6*1000g 18 500 Kč

Peptone

Kód Množství Cena bez DPH

PEP01 250g 1 400 Kč

PEP02 500g 2 250 Kč

PEP03 1000g 4 250 Kč

PEP04 6*1000g 23 500 Kč

Popis produktu

Soya Peptone is a papaic digest of defatted soybean flour and is a well-balanced source of essential amino acids, carbohydrates and vitamins in cell cultures.

Soya Peptone is used for growth of a wide variety of bacteria and yeasts in cell cultures and is often combined with Tryptone or Peptone for a rapid and abundant growth of cells.

This plant peptone is classified animal-free by Formedium Ltd. Based on the manufacturing protocol, we attest that no animal raw materials are prescribed for use in the production of this product, nor are any of the raw materials derived from animal products.

Soya Peptone is classified animal free, GMO free (according to the European Directive 2001/18/CE)

Store dry at room temperature

Popis produktu

Peptone is a spray dried powder, manufactured by a controlled enzymatic hydrolysis of animal tissue. The most commonly used enzymes are pepsin, papain and pancreatin. The latter containing trypsin.

Pepsin will cut the peptide chain anywhere there is a phenylalanine or leucine bond.

Papain cuts in the peptide chain adjacent to arginine, lysine, phenylalanine and glycine. Pancreatin has its action at arginine, lysine, tyrosine, tryptophan, phenylalanine and leucine bonds.

The tissues are hydrolysed to produce straw coloured peptones which are highly nutritious and clearly soluble in water. Peptones contain a mix of peptides, free amino acids and growth factors.

Due to the low sodium chloride concentration Formedium™Pepton is well suited for cultivation of yeast cells.

Store dry at room temperature

CAS Number 73049-73-7

PDB

Kód Množství Cena bez DPH

PDB0101 100g 500 Kč

PDB0102 1000g 2 900 Kč

PDB0103 5000g 12 000 Kč

PDA

Kód Množství Cena bez DPH

PDA0101 100g 750 Kč

PDA0102 1000g 3 250 Kč

PDA0103 5000g 14 000 Kč

Popis produktu

Suspend 24 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Formula g/l

Potato extrakt 4

Glucose 20

Popis produktu

Suspend 41 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Potato extrakt 4

Glucose 20

Agar 17

Potato extract

Kód Množství Cena bez DPH

PTE01 250g 1 500 Kč

PTE02 500g 2 200 Kč

PTE03 1000g 3 500 Kč

PTE04 6*1000g 18 500 Kč

Casamino acids

Kód Množství Cena bez DPH

CAS01 250g 1 800 Kč

CAS02 500g 2 700 Kč

CAS03 1000g 5 400 Kč

CAS04 6x1000g 29 750 Kč

Popis produktu

Potato extract is a mixture of potato proteins, manufactured by controlled enzymatic hydrolysis. The extract is an excellent nitrogen source for bacteria, yeasts and fungi. Potato extract is rich in vitamins and minerals and supports a vigorous growth of micro-organisms.

Store dry at room temperature

Popis produktu

Casamino Acids are manufactured by a controlled acid hydrolysis of casein . Hydrolysis is not completed until all the nitrogen in the casein is converted to amino acids or other compounds of relative chemical simplicity. As a result of the acid hydrolysis process all vitamins and growth factors present in casein are destroyed.

Due to the low sodium chloride concentration Formedium™Casamino Acids are well suited for cultivation of yeast cells.

Tryptone

Kód Množství Cena bez DPH

TRP01 250g 1 400 Kč

TRP02 500g 2 400 Kč

TRP03 1000g 4 200 Kč

TRP04 6*1000g 23 500 Kč

Yeast potassium nitrate nitrogen base

Kód Množství Cena bez DPH

CYN0301 100g 1 700 Kč

CYN0302 250g 3 400 Kč

CYN0305 500g 6 100 Kč

CYN0310 1000g 9 800 Kč

Popis produktu

Store dry at room temperature

CAS 91079-40-2

Popis produktu

Suspend 2.7 gram powdered medium in 1 litre distilled water
Store dry at room temperature

Yeast Potassium Nitrate Nitrogen Base contains Potassium Nitrate as a sole nitrogen source to test nitrogen assimilation. The addition of a carbon source is required. Histidine, Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base.

Yeasts which have grown on a rich medium like Yeast Agar may carry a reserve of nitrogen in the form of protein. To avoid errors due to this reserve of nitrogen it is advised to make two serial transfers in the medium. Seven days after the first inoculation the second inoculation is made in the same medium. If a positive result is obtained after seven days, the yeast cells assimilates nitrate as sole nitrogen source.

SD broth 2% glucose

Kód Množství Cena bez DPH

CSM0201 100g 1 250 Kč

CSM0202 250g 2 300 Kč

CSM0205 500g 4 300 Kč

CSM0210 1000g 7 800 Kč

SD broth 2% galactose

Kód Množství Cena bez DPH

CSM0401 100g 2 200 Kč

CSM0402 250g 3 900 Kč

CSM0405 500g 7 500 Kč

CSM0410 1000g 14 500 Kč

Popis produktu

Suspend 26.9 gram powdered medium in 1 litre distilled water

Store dry at room temperature

SD Broth / Glucose containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.

Popis produktu

Suspend 26.9 gram powdered medium in 1 litre distilled water

Store dry at room temperature

SD Broth / Galactose containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.

SD broth 2% raffinose

Kód Množství Cena bez DPH

CSM0601 100g 2 800 Kč

CSM0602 250g 5 500 Kč

CSM0605 500g 11 000 Kč

CSM0610 1000g 20 000 Kč

SD broth 3.3% succinate

Kód Množství Cena bez DPH

CSM0801 100g 1 250 Kč

CSM0802 250g 2 500 Kč

CSM0805 500g 4 500 Kč

CSM0810 1000g 8 500 Kč

Popis produktu

Suspend 26.9 gram powdered medium in 1 litre distilled water

Store dry at room temperature

SD Broth / Raffinose containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.

Popis produktu

Suspend 57.9 gram powdered medium in 1 litre distilled water

SD Broth / Succinate containing Yeast Nitrogen base without Amino Acids and glucose. Agar has been omitted from the original formulation.

SD broth 2% glucose

without phosphate KH2PO4

Kód Množství Cena bez DPH

CSM1201 100g 1 250 Kč

CSM1202 250g 2 250 Kč

CSM1205 500g 4 000 Kč

CSM1210 1000g 7 500 Kč

SD broth 2% galactose

1% raffinose

Kód Množství Cena bez DPH

CSM1001 100g 2 600 Kč

CSM1002 250g 5 600 Kč

CSM1005 500g 9 500 Kč

CSM1010 1000g 16 750 Kč

Popis produktu

Suspend 25.9 gram powdered medium in 1 litre distilled water

Store dry at room temperature

SD Broth / 2% Glucose w/o Phosphate (KH2PO4) is based upon Yeast Nitrogen base without Amino acids and including glucose. The absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines.

Popis produktu

Suspend 36.9 gram powdered medium in 1 litre distilled water

Store dry at room temperature

SD Broth / Galactose and Raffinose containing Yeast Nitrogen base without Amino Acids, galactose and raffinose. Agar has been omitted from the original formulation.

YPD Agar

Kód Množství Cena bez DPH

CCM0102 250g 1 200 Kč

CCM0105 500g 1 600 Kč

CCM0110 1000g 2 900 Kč

YEP Agar

Kód Množství Cena bez DPH

CCM0302 250g 1 200 Kč

CCM0305 500g 2 100 Kč

CCM0310 1000g 3 500 Kč

Popis produktu

Suspend 70 gram powdered medium in 1 litre distilled water

Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Glucose 20

Agar 20

Popis produktu

Suspend 50 gram powdered medium in 1 litre distilled water

Store dry at room temperature.

YEP Medium is based upon YPD but is without dextrose and can be used as a base for making YPD media with an alternate carbon source.

To make YEPG-Medium add 38 ml glycerol per litre YEP medium. Glycerol is a non-fermentable carbon source. Respiratory deficient and pet mutants will not grow on YPEG.

Formula g/l

Yeast extract 10

Peptone 20

Agar 20

YPAD Agar

Kód Množství Cena bez DPH

CCM0502 250g 1 200 Kč

CCM0505 500g 2 100 Kč

CCM0510 1000g 3 500 Kč

YPL Agar with Lactose

Kód Množství Cena bez DPH

YPLA01 250g 1 200 Kč

YPLA02 500g 2 400 Kč

YPLA03 1000g 3 700 Kč

Popis produktu

Suspend 70 gram powdered medium in 1 litre distilled water

Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Glucose 20

Adenine Sulfate 0.04

Agar 20

Popis produktu

Suspend 70 gram powdered medium in 1 litre distilled water

Store dry at room temperature.

Formula g/l

Yeast extract 10

Peptone 20

Lactose 20

Adenine Sulfate 0.04

Agar 20

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